Fig 1: Flow cytometry and immunofluorescence of Sca1+ FAPs and ITGA7+ MuSCs.(A) Scatter plots of Sca1+ FAPs (from 45-d old wt mice) stained with antibodies raised against CD140a-APC (PDGFRa) (left panel). Scatter plots of ITGA7+ MuSCs (from 45-d-old wt mice) stained with antibodies raised against ITG7-APC and Pax7-FITC (right panel). (B) Percentage of Sca1+ FAPs expressing CD140a (PDGFRa) (left table). Percentage of ITGA7+ MuSCs co-expressing ITGA7 and Pax7 (left table). Data from three independent cell preparations are shown. (C) Representative immunofluorescence showing the expression of CD140a (PDGFRa) in FAPs and the expression of Pax7 and MyoD in MuSCs.
Fig 2: Transcriptomic profiles of ITGA6bright cells reveal their peripheral glial lineage. (A) Volcano plot showing the differentially expressed genes between ITGA6bright and GFPneg ITGA6neg cells (p-value < 0.05, absolute log2 Fold Change >1). (B) GOChord plot of the top GO terms within the biological process (BP) subontology. The upregulated genes in ITGA6bright cells are linked to their assigned pathways by ribbons and ordered according to log2 of the fold-change values (from high to low) represented by a color gradient indicated by the logFC bar. (C) Heatmap of selective ligand-, receptor-, and transcription factor (TF)-coding genes highly expressed in ITGA6bright cells. (D) Flow cytometry results for the expression of ITGA6, MCAM, ITGA7, and ITGA1 on ITGA6bright, ITGA6dim, and ITGA6neg cells. *, compared with ITGA6-neg, *p < 0.05; **p < 0.01; ****p < 0.0001; #, compared with ITGA6-dim, #p < 0.05; ####p < 0.0001. (E) Regulatory network analysis showing the predicted TFs (octagonal nodes) among the enriched genes (rounded nodes) in ITGA6bright cells.
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